rabbit monoclonal antibody against human grp78  (Cell Signaling Technology Inc)

Journal: Liver Research

Article Title: Combination of brefeldin A and tunicamycin induces apoptosis in HepG2 cells through the endoplasmic reticulum stress-activated PERK-eIF2α-ATF4-CHOP signaling pathway

doi: 10.1016/j.livres.2025.01.004

Figure Lengend Snippet: Antibodies used for Western blots.

Article Snippet: Rabbit anti-human BiP/GRP78 , Protein Tech, China , 66574-1-Ig , 1:1000.

Techniques: Western Blot

Journal: International Journal of Oncology

Article Title: Galectin-1 binds GRP78 to promote the proliferation and metastasis of gastric cancer

doi: 10.3892/ijo.2022.5431

Figure Lengend Snippet: Associations between Gal-1 expression and clinicopathological features of 80 patients with gastric cancer.

Article Snippet: The cells were permeabilized with 0.1% Triton X-100 for 15 min. After blocking with 5% bovine serum albumin (Beyotime Institute of Biotechnology) at room temperature for 60 min, the cells were incubated with the following antibodies for 60 min at 37°C: Rabbit anti-human GRP78 (1:300; cat. no. 11587-1-AP) and mouse anti-human Gal-1 (1:100; cat. no. 60223-1-Ig; both from ProteinTech Group, Inc.).

Techniques: Expressing

Journal: International Journal of Oncology

Article Title: Galectin-1 binds GRP78 to promote the proliferation and metastasis of gastric cancer

doi: 10.3892/ijo.2022.5431

Figure Lengend Snippet: Expression of GRP78 in gastric cancer with different clinicopathological variables using our own cohort.

Article Snippet: The cells were permeabilized with 0.1% Triton X-100 for 15 min. After blocking with 5% bovine serum albumin (Beyotime Institute of Biotechnology) at room temperature for 60 min, the cells were incubated with the following antibodies for 60 min at 37°C: Rabbit anti-human GRP78 (1:300; cat. no. 11587-1-AP) and mouse anti-human Gal-1 (1:100; cat. no. 60223-1-Ig; both from ProteinTech Group, Inc.).

Techniques: Expressing

Journal: International Journal of Oncology

Article Title: Galectin-1 binds GRP78 to promote the proliferation and metastasis of gastric cancer

doi: 10.3892/ijo.2022.5431

Figure Lengend Snippet: GRP78 is associated with Gal-1. (A) The interaction protein network of Gal-1 was revealed by the BioGRID database ( https://thebiogrid.org/ ). (B) GRP78 mRNA expression in GC (n=408) and average (n=211) samples were evaluated using the online tool GEPIA. (C) The expression level of Gal-1 mRNA is independent of GRP78 mRNA in The Cancer Genome Atlas database. (D and E) Gal-1 physically interacted with GRP78. The endogenous proteins from HGC-27 cells were immunoprecipitated with IgG or antibodies against Gal-1 and GRP78, followed by western blot analysis and cell lysis for input. (F) Immunofluorescence colocalization of Gal-1 and GRP78 in GC cells (scale bar, 10 µ m). (G) A western blot assay was performed to detect the GRP78 protein levels in the HGC-27 cells, knocking-down Gal-1. (H) Western blot analysis was performed to detect the GRP78 protein levels in the AGS cells overexpressing Gal-1. * P<0.05. GRP78, glucose-regulated protein 78; Gal-1, galectin-1; GC, gastric cancer; sh-, short hairpin; OE, overexpression.

Article Snippet: The cells were permeabilized with 0.1% Triton X-100 for 15 min. After blocking with 5% bovine serum albumin (Beyotime Institute of Biotechnology) at room temperature for 60 min, the cells were incubated with the following antibodies for 60 min at 37°C: Rabbit anti-human GRP78 (1:300; cat. no. 11587-1-AP) and mouse anti-human Gal-1 (1:100; cat. no. 60223-1-Ig; both from ProteinTech Group, Inc.).

Techniques: Expressing, Immunoprecipitation, Western Blot, Lysis, Immunofluorescence, Over Expression

Journal: International Journal of Oncology

Article Title: Galectin-1 binds GRP78 to promote the proliferation and metastasis of gastric cancer

doi: 10.3892/ijo.2022.5431

Figure Lengend Snippet: (A) The IHC assay of Gal-1 and GRP78 expression in GC tissues and corresponding adjacent normal tissues was performed, and the representative images were shown. (B) Qualification of GRP78 staining in TMAs described in panel A. The graph depicts the total score, the multiplication product of staining intensity and the percentage of stained cells. (C) Statistical analysis of the relationship between the expression level of GRP78 and Gal-1 (P-value: Spearman's correlation coefficient). (D) Kaplan-Meier overall survival curves for all 80 patients with GC stratified by high and low expression of GRP78. ** P<0.01. Gal-1, galectin-1; GRP78, glucose-regulated protein 78; IHC, immunohistochemical; GC, gastric cancer.

Article Snippet: The cells were permeabilized with 0.1% Triton X-100 for 15 min. After blocking with 5% bovine serum albumin (Beyotime Institute of Biotechnology) at room temperature for 60 min, the cells were incubated with the following antibodies for 60 min at 37°C: Rabbit anti-human GRP78 (1:300; cat. no. 11587-1-AP) and mouse anti-human Gal-1 (1:100; cat. no. 60223-1-Ig; both from ProteinTech Group, Inc.).

Techniques: Expressing, Staining, Immunohistochemical staining

Journal: International Journal of Oncology

Article Title: Galectin-1 binds GRP78 to promote the proliferation and metastasis of gastric cancer

doi: 10.3892/ijo.2022.5431

Figure Lengend Snippet: Tumor-promoting effect of Gal-1 is dependent on the upregulation of GRP78. (A) The knockdown and overexpression efficiency of GRP78 in AGS and HGC-27 cells was confirmed by western blot analysis. (B) Colony formation assay revealed that ectopic GRP78 expression restored the number of cell colonies in shGal-1HGC-27 cells. (C) Ectopic GRP78 expression promoted migration and invasion in shGal-1 HGC-27 cells. (D) Colony formation assay showed that lentivirus-mediated knockdown of GRP78, and ectopic Gal-1 expression inhibited cell growth of AGS cells. (E) Knockout GRP78 inhibited migration and invasion in OE-Gal-1 AGS cells. * P<0.05, ** P<0.01, *** P<0.001 and **** P<0.0001. Gal-1, galectin-1; GRP78, glucose-regulated protein 78; sh-, short hairpin; OE, overexpression.

Article Snippet: The cells were permeabilized with 0.1% Triton X-100 for 15 min. After blocking with 5% bovine serum albumin (Beyotime Institute of Biotechnology) at room temperature for 60 min, the cells were incubated with the following antibodies for 60 min at 37°C: Rabbit anti-human GRP78 (1:300; cat. no. 11587-1-AP) and mouse anti-human Gal-1 (1:100; cat. no. 60223-1-Ig; both from ProteinTech Group, Inc.).

Techniques: Knockdown, Over Expression, Western Blot, Colony Assay, Expressing, Migration, Knock-Out